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Image Search Results
Journal: Frontiers in Immunology
Article Title: Multidimensional Analysis Integrating Human T-Cell Signatures in Lymphatic Tissues with Sex of Humanized Mice for Prediction of Responses after Dendritic Cell Immunization
doi: 10.3389/fimmu.2017.01709
Figure Lengend Snippet: Functional memory T responses against pp65 after induced dendritic cells expressing pp65 (iDCpp65) immunization. (A) T cells isolated from lymph nodes of immunized mice ( n = 3) were re-stimulated in vitro with either induced DC (iDC) or iDCpp65. Absolute T cell numbers before (white bars) and 7 days after (black bars) stimulation are shown. (B) Relative fold increase of cells populations before and 7 days after coculture with iDC (gray bars) and iDCpp65 group (black bar). (C) CD8 + and (D) CD4 + T lymphocytes expanded after coculture with iDC ( n = 3) or iDCpp65 ( n = 3) were left unstimulated (white bars) or re-stimulated with a Wilms Tumor 1 (WT1) peptide pool (gray bars) or pp65-peptide pool (black bars). Frequency of cells producing IFN-γ is shown. (E) T cells expanded with iDC (four cultures per group) or iDCpp65 (six cultures per group) were cocultured with an artificial antigen-presenting cell (aAPC) on an ELISPOT plate. The aAPCs were ether not loaded (white bars), loaded with WT1 peptides (light gray bars), loaded with pp65-peptides (dark gray bars), or transduced for endogenous pp65 expression and loading (black bars). Mean absolute number of IFN-γ-spots and * p < 0.05 (analysis of variance), † p < 0.05 ( F -test) are indicated.
Article Snippet: A two-way analysis of variance was performed to analyze the results of the
Techniques: Functional Assay, Expressing, Isolation, In Vitro, Wilms Tumor Assay, Enzyme-linked Immunospot